Recombinant Chymotrypsin

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Recombinant Chymotrypsin Animal Protein Free, No Virus Contaminant DESCRIPTION Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is same as that human chymotrypsin. The recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in the pH range of 7.0–9.0. This sequencing grade enzyme can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel. ADVANTAGES ● Animal origin free The use of recombinant chymotrypsin eliminates the content of other proteins, the risk of virus presence, and other potential adventitious agents found in animal-derived chymotrypsin. ● Stable A sterile recombinant chymotrypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage. ● High purity 1)Recombinant Chymotrypsin provides increased specificity and eliminates contaminating activities found in enzymes purified from pancreas. 2) No anion trypsin or cation trypsin. 3) One single main band at 25kD by SDS-PAGE. SPECIFICATION Serine endopeptidase that specifically hydrolyzes peptide bonds at the C-termini of Tyr, Phe, and Trp. Leu, Met, Ala, Asp, and Glu are cleaved at a lower rate. Acts also upon amides and esters of susceptible amino acids. APPLICATION Hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. Suitable for peptide mapping, fingerprinting, and sequence analysis. For peptide digestion, use a ratio (w/w) of approximately 1:60 for chymotrypsin:peptide. Perform peptide digests in 100 mM Tris HCl containing 10 mM CaCl2, pH 7.8, at 30 °C. Self digestion may occur if temperatures above 37 °C are used. A known peptide such as melittin should be used as a control for all experiments. MAIN FEATURES Source E. Coli Purified by HPLC Physical form White lyophilized powder Specific activity ≥1000 unit/mg pro. Purity > 95% PRODUCT INFORMATION Product Cat. No. Specific activity Packaging Manufacturer Recombinant Chymotrypsin RCT10 ≥1000 unit/mg pro. 1mg,100mg,1g, bulk YaxinBio UNIT DEFINITION: One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C. RELATED PRODUCT: Recombinant Trypsin,V8

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Shanghai Yaxin Biotechnology Co., Ltd.

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