Human Hyaluronic acid (HA) ELISA Kit

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Human Hyaluronic acid (HA) ELISA Kit Purpose This kit allows for the determination of HA concentrations in Human serum, blood plasma,and other biological fluids. Principle of the assay The kit assay Human HA level in the sample，use Purified Human HA antibody to coat microtiter plate wells, make solid-phase antibody, then add HA to wells, Combined HA antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HA in the samples is then determined by comparing the O.D. of the samples to the standard curve. Materials provided with the kit Materials provided with the kit 48 determinations 96 determinations Storage User manual 1 1 Closure plate membrane 2 2 Sealed bags 1 1 Microelisa stripplate 1 1 2-8℃ Standard：360ng/L 0.5ml×1 bottle 0.5ml×1 bottle 2-8℃ Standard diluent 1.5ml×1 bottle 1.5ml×1 bottle 2-8℃ HRP-Conjugate reage 3ml×1 bottle 6ml×1 bottle 2-8℃ Sample diluent 3ml×1 bottle 6ml×1 bottle 2-8℃ Chromogen Solution A 3ml×1 bottle 6ml×1 bottle 2-8℃ Chromogen Solution B 3ml×1 bottle 6ml×1 bottle 2-8℃ Stop Solution 3ml×1 bottle 6ml×1 bottle 2-8℃ wash solution （20ml×20 fold） ×1bottle （20ml×30 fold） ×1bottle 2-8℃

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Glory Science Co., Ltd.

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