Goat Interferon γ(IFN-γ) ELISA Kit

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Goat Interferon γ(IFN-γ) ELISA Kit Purpose This kit allows for the determination of IFN-γ concentrations in Goat serum, blood plasma, and other biological fluids. Principle of the assay The kit assay Goat IFN-γ level in the sample，use Purified Goat IFN-γ antibody to coat microtiter plate wells, make solid-phase antibody, then add IFN-γ to wells, Combined IFN-γ antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IFN-γ in the samples is then determined by comparing the O.D. of the samples to the standard curve. Materials provided with the kit Materials provided with the kit 48 determinations 96 determinations Storage User manual 1 1 Closure plate membrane 2 2 Sealed bags 1 1 Microelisa stripplate 1 1 2-8℃ Standard：45ng/L 0.5ml×1 bottle 0.5ml×1 bottle 2-8℃ Standard diluent 1.5ml×1 bottle 1.5ml×1 bottle 2-8℃ HRP-Conjugate reage 3ml×1 bottle 6ml×1 bottle 2-8℃ Sample diluent 3ml×1 bottle 6ml×1 bottle 2-8℃ Chromogen Solution A 3ml×1 bottle 6ml×1 bottle 2-8℃ Chromogen Solution B 3ml×1 bottle 6ml×1 bottle 2-8℃ Stop Solution 3ml×1 bottle 6ml×1 bottle 2-8℃ wash solution （20ml×20 fold） ×1bottle （20ml×30 fold） ×1bottle 2-8℃

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Glory Science Co., Ltd.

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