Troponin I Serum Test

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Cardiac troponin I (cTnI) is a cardiac muscle protein with a molecular weight of 22.5 kilodaltons. Together with troponin T (TnT) and troponin C (TnC), TnI forms a troponin complex in heart to play a fundamental role in the transmission of intracellular calcium signal acting-myosin interaction. The human cTnI has an additional amino acid residues on its N-terminal that are not exist on the skeletal forms thus making cTnI a specific marker for indicating cardiac infarction. CTnI is released rapidly into blood after the onset of acute myocardial infarction (AMI). Its release pattern is similar to CK-MB (4-6 hours after the onset of AMI). However, CK-MB level returns to normal after 36-48 hours, while levels of cTnI remains elevated for up to 6-10 days. The level of cTnI is very low in normal healthy people, and not detected in patients with skeletal muscle injury. Therefore, cTnI is a specific marker for diagnosis of AMI. Troponin I Rapid Test Card is a sandwich immunoassay. When serum sample is added to sample pad, it moves through the conjugate pad and mobilizes gold anti-cTnI conjugate that are coated on the conjugate pads. The mixture moves along the membrane by capillary action and reacts with anti-cTnI antibody that is coated on the test region of troponin I test strip. If Troponin I is present at 0.3ng/ml or greater, the result is the formation of a colored band in the test region. If there is no cTnI in the sample, the area will remain colorless. The sample continues to move to the control area and forms a pink to purple color, indicating the test is working and the result is valid. Either whole blood, plasma or serum may be used as samples for this procedure. For the whole blood or plasma procedure, collect blood in a tube containing heparin as the anticoagulant. If serum samples are to be used, collect blood in a tube without anticoagulant and allow to clot. Since cardiac proteins are relatively unstable, it is recommended that fresh samples be used as soon as possible to collect critical patient information. Heat inactivation of samples may lead to hemolysis or protein denaturation and therefore should be avoided. Whole blood samples should be tested within 4 hours of collection. If specimens are to be stored, the red blood cells should be removed. Whole blood samples should not be frozen. Plasma or serum samples may be refrigerated for 24 hours at 2-8 °C. If plasma or serum samples are to be stored for more than 24 hours, they should be stored frozen at -20 °C or below.

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Biochema Ltd.

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