Bovine Epithelial cell adhesion molecule,EpCAM ELISA Kit

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MER & CIEL is one of the core brands of Putus Macromolecular Sci. & Tech.Ltd. We offer kits, reagents, service and technical support that enable researchers and clinicians to test and diagnose molecules and proteins of interest. Applying innovative techniques including high-density cell culture, large-scale protein production, and Mw-cutoff spin column purification of protein, MER & CIEL has prepared and commercialized over thousands of antigens and antibodies of high purity and specificity. Presently we offer highly specific monoclonal and polyclonal antibodies, antibody pairs, and high quality ELISA kits. We also offer software for massive data analysis of ELISA. We provide professional service and technical support to our customers. Product Type Bovine Epithelial cell adhesion molecule,EpCAM ELISA Kit code number BE020 Species Bovine Price $557 Specification 96T Target Name Epithelial cell adhesion molecule Principles of the Test This type of assay is called “sandwich” assay because the analyte to be measured is bound between the coating (capture) antibodies and the detection antibodies. The sandwich format is sensitive and robust. Here, monoclonal Bovine EpCAM antibodies,showing high specificity toward a single epitope, are used as capture antibody to provide specificity and allow fine detection and quantification of antigens; biotin-conjugated monoclonal Bovine EpCAM antibodies, recognizing epitope(s) that are not recognized by capture antibodies, are used as detection antibodies. When the antigen binds to the capture antibody, the epitope recognized by the detection antibody must not be obscured or altered. Capture and detection antibodies that do not interfere with one another and can bind simultaneously are considered a matched pair and are suitable for developing a sandwich ELISA. The biotin-conjugated detection antibodies are further reacted with streptavidin-conjugated HRP-linked enzyme, which can develop a color upon the addition of TMB substrate. Format Colorimetric ELISA with TMB substrate for 96-well microplate Measurement Absorbance 450 nm as the primary wave length (optionally 620 nm as the reference wave length) Specificity No cross-reaction with circulating factors of the immune systeme Spike Recovery Average recovery ranged from80% to 110% with an overall mean recovery of101%. Performance Certified Yes. Well-to-well. CV < 5% Product description Content 1) Precoated 96-well Strip Plate with monoclonal antibody 2) 1 bottle Sample Diluent; 3) 1 vial Standard ; 4) 1 vial biotin-conjugated antibody; 5) 1 vial Streptavidin-HRP; 6) 1 bottle Conjugate Diluent; 7) 1 bottle wash buffer 8) 1bottle TMB (tetramethyl-benzidine) substrate Solution; 9) 1 bottle Stop Solution ; 10) 1 Adhesive Plate Cover. Total Assay Time 4h Sample Volume 50ul Intended Use For research use only. Not for diagnostic use Delivery date within three days of delivery

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Putus Macromolecular Sci. & Tech.ltd

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