Nitrate/Nitrite Assay Kit

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Nitrate/Nitrite Assay Kit
Posting date : Apr 19, 2013
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28-
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Product Description REAGEN™ Nitrate/Nitrite Assay Kit is a cuvette-based enzymatic assay for the determination of Nitrate/Nitrite across a variety of food samples. The kit uses a spectrophotometric, kinetic assay to detect the enzymatic degradation of Nitrate/Nitrite directly from food samples. The unique features of the kit are: 1.High sensitivity and low detection limit (0.02 ppm) 2.High reproducibility 3.A rapid (45 minute), robust enzyme-based assay Procedure Overview REAGEN™ Nitrate/Nitrite Assay Kit uses a coupled enzymatic reaction scheme to detect nitrate and/or nitrite in food samples: nitrate in the sample is converted by nitrate reductase enzyme to make nitrite. The nitrite then reacts with sulfanilamide and N-(1-naphthyl)-ethylene-diamine to form a red diazo dye. This dye, measured at 540 nm, is proportional to the level of nitrate and/or nitrite in the sample. The absorbance of each sample at 540 nm is measured using a spectrophotometer. The concentration of nitrate /nitrite in each sample is then directly determined from the diazo dye absorbance at 540 nm within 45 minutes.

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