Melamine ELISA Test Kit

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Product Description REAGEN™Melamine ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of melamine in milk, milk powder, infant formula, meat, fish, feed, chocolate and other food samples. The unique features of the kit are:  High recovery (>80%),rapid(10-40 minutes),and cost-effective extraction methods.  High sensitivity(5ng/g or ppb)and low detection limit for liquid milk (0.2ppm).  A quick ELISA assay(less than 2 hours regardless of number of samples).  High reproducibility. Procedure Overview The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin and enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The enzyme-conjugate, will bind with the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample. Kit Contents, Storage and Shelf Life REAGEN™Melamine ELISA Test Kit has the capacity for 96 determinations or testing 、of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored. Kit Contents Amount Storage Melamine Coated Plate 1 x 96-well Plate (8 wells x 12 strips) 2-8°C Melamine Standards: Negative control (white cap tube) 5 ng/mL (yellow cap tube) 20 ng/mL (orange cap tube) 50 ng/mL (pink cap tube) 100 ng/mL (purple cap tube) 200 ng/mL (blue cap tube) 100 µg/mL (spiking, optional, red cap tube) 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 2-8°C * Melamine Antibody 8 mL 2-8°C * 100X HRP Conjugate 100 µL 2-8°C * HRP Conjugate Diluent 10 mL 2-8°C 20X Wash Solution 28 mL 2-8°C Stop Buffer 20 mL 2-8°C TMB Substrate 12 mL 2-8°C 10 X Melamine Sample Extraction Buffer 20 mL X 2 2-8°C 1 X Melamine Clean Up Buffer 20 mL 2-8°C 1X Melamine Sample Balance Buffer 20 mL 2-8°C 10X Melamine Sample Dilution Buffer 20 mL 2-8°C *If you are not planning to use the kit for over 1 month, store Melamine Standards, Melamine Antibody and 100X HRP Conjugate at -20°C or in a freezer. Sensitivity (Detection Limit) Sample Type Detection Limit (g/g or ppb) Feed 0.5 ppm Meat/Fish 0.2 ppm Milk 0.2 ppm Milk Powder/Infant Formula 0.4 ppm Chocolate 0.4 ppm

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reagen llc

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