Sulfonamide ELISA Test Kit

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Product Description REAGEN™Sulfonamide ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of sulfonamide in feed, fish, eggs, honey, meat (beef, chicken and pork), milk, milk powder, serum, shrimp and urine. The unique features of the kit are:  Rapid extraction method for various samples with high recovery (>80%).  High sensitivity (0.5 ppb) and low detection limit (0.25 ppb for shrimp).  A quick ELISA assay (less than 2 hours regardless of number of samples).  High reproducibility. Procedure Overview The method is based on a competitive colorimetric ELISA assay. The plate wells are coated with capture antibodies directed against anti- sulfonamide antibodies. Sulfonamide standards or sample solution, sulfonamide–HRP conjugate and anti-sulfonamide antibodies are added. Free sulfonamide and sulfonamide-HRP Conjugate compete for the sulfonamide antibody binding sites. At the same time, the anti- sulfonamide antibodies are also bound by the immobilized capture antibodies. Any unbound sulfonamide-HRP Conjugate is then removed in a washing step. The TMB Substrate is added to the wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample. Kit Contents, Storage and Shelf Life REAGEN™ Sulfonamide ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C *. The shelf life is 12 months when the kit is properly stored. Kit Contents Amount Storage Sulfonamide Plate 1x96-well Plate(8wellsx12 strips) 2-8C SMX Standards: Negative control (white cap tube) 0.5 ng/mL (yellow cap tube) 1.5 ng/mL (orange cap tube) 5.0 ng/mL (pink cap tube) 15 ng/mL (purple cap tube) 50 ng/mL (blue cap tube) 1000 ng/mL(spiking, optional, red cap tube) 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 2-8C Sulfonamide Antibody (powder) 2 tubes 2-8C * Antibody Dilution Buffer 8 mL 2-8C * Sulfonamide-HRP Conjugate 6 mL 2-8C * 10X Sample Extraction Buffer E 25 mL 2-8C 20X Wash Solution ** 28 mL 2-8C Stop Buffer ** 14 mL 2-8C TMB Substrate ** 12 mL 2-8C Concentrate of Tissue Extraction Buffer 60 g 2-8C Tissue Clean Up Mix 12 g 2-8C Honey Extraction Buffer (Optional) 3 x 28 mL 2-8C * If you are not planning to use the kit for over 1 month, store Sulfonamide Antibody, Antibody Dilution Buffer and Sulfonamide -HRP Conjugate at -20C or in a freezer. Sensitivity Sample Type Detection Limit (ng/g or ppb) Feed 2.5 Egg 0.25 Fish/Shrimp/Meat/Liver/Kidney 1.0 Honey 1.0 Milk 2.5 Milk Powder 2.0 Serum 2.5 Shrimp 0.25 (Rapid Extraction Method) Urine 2.5 Water 0.25

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reagen llc

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