Nitrofurazone(SEM)ELISA Test Kit | Global Trade leader ecrobot

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Nitrofurazone(SEM)ELISA Test Kit

Posting date : Nov 23, 2011

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Product Description REAGEN™ Nitrofurazone (SEM) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Nitrofurazone in feed, fish, eggs, honey, meat (beef, chicken and pork), milk, milk powder, serum, shrimp and urine. The unique features of the kit are:  high recovery (75%-95%), and cost-effective extraction methods for various samples.  High sensitivity (0.05 ng/g or ppb) and low detection limit (0.1 ng/g or ppb) for various samples.  A quick ELISA assay (less than 1.5hours regardless of number of samples).  High reproducibility. Procedure Overview The method is based on a competitive colorimetric ELISA assay. The SEM antibody has been coated in the plate wells. During the analysis, sample is added along with the SEM-horseradish peroxidase (SEM-HRP) conjugate. If the SEM residue is present in the sample, it will compete for the SEM antibody, thereby preventing the SEM-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the SEM residue concentration in the sample. Kit Contents, Storage and Shelf Life REAGEN™ Nitrofurazone (SEM) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C*. The shelf life is 12 months when the kit is properly stored. Kit Contents Amount Storage SEM Antibody-Coated Plate 96-well plate (8 wells x 12 strips) 2-8C SEM Standards: Negative control (white cap tube) 0.05 ng/mL (yellow cap tube) 0.2ng/mL (orange cap tube) 0.8 ng/mL (pink cap tube) 3.2 ng/mL (purple cap tube) 9.6 ng/mL (blue cap tube) 10 ng/mL (spiking, optional, red cap tube) 1.8 mL 1.8 mL 1.8 mL 1.8 mL 1.8 mL 1.8 mL 1.8 mL 2-8C SEM- HRP Conjugate 6 mL 2-8C * 10X Sample Extraction Buffer ** 25 mL 2-8C 20X Wash Solution ** 28 mL 2-8C Stop Buffer ** 20 mL 2-8C TMB Substrate ** 12 mL 2-8C 50 mM 2-Nitrobenzaldehyde ** 1.8 mL 2-8C * If you are not planning to use the kit for over 1 month, store SEM- HRP Conjugate at -20C or in a freezer. Sensitivity Sample Type Detection Limit (ng/g or ppb) Feed 0.2 Fish/Shrimp/Meat 0.1 Egg/Egg Powder 0.1 Honey 0.1 Milk 0.1 Serum 0.1 Urine 0.1