Clenbuterol ELISA Test Kit | Global Trade leader ecrobot

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Clenbuterol ELISA Test Kit

Posting date : Nov 23, 2011

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CHEMICAL | Miscellane

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Product Description REAGEN™Clenbuterol ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of clenbuterol and other related ß-agonists in feed, meat/liver/kidney, milk and urine. REAGEN™Clenbuterol ELISA Test Kit enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organizations, to detect clenbuterol in various sample types in response to customer concerns about food safety. The unique features of the kit are:  Rapid (10 - 30 minutes), and organic reagent-free extraction method for various samples with high recovery (75 - 95%).  High sensitivity (0.05 ng/g or ppb) and low detection limit (0.025 ng/g or ppb for meat/tissue, 0.05 ng/g or ppb for urine and 0.2 ng/g or ppb for feed).  A quick ELISA assay (less than 2 hours regardless of number of samples.  High reproducibility. Procedure Overview The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample. Kit Contents, Storage and Shelf Life Kit Contents Amount Storage Clenbuterol-coated Microtiter Plate 1 x 96-well plate (8 wells x 12 strips) 2-8C Clenbuterol Standards: Negative control (white cap tube) 0.05 ng/mL (yellow cap tube) 0.15 ng/mL (orange cap tube) 0.5 ng/mL (pink cap tube) 1.5 ng/mL (purple cap tube) 4.5 ng/mL (blue cap tube) 10 ng/mL (spiking, optional, red cap tube) 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 2-8C Clenbuterol Antibody #1 15 mL 2-8C * 100X HRP-Conjugated Antibody #2 250 L 2-8C * Antibody #2 Diluent ** 20 mL 2-8C 200X Sample Extraction Buffer ** 25 mL 2-8C 20X Wash Solution ** 28 mL 2-8C Stop Buffer ** 20 mL 2-8C TMB Substrate ** 12 mL 2-8C Sample Balance Buffer ** 0.5 mL 2-8C 10X PBS ** 25 mL 2-8C * If you are not planning to use the kit for over 1 month, store Clenbuterol Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20C or in a freezer. Sensitivity Sample Type Detection Limit (ng/g or ppb) Feed 0.2 (Rapid Extraction Method) Meat/Liver/Kidney 0.025 (Organic Reagent Extraction Method) Milk 0.25 Urine 0.05