Melamine ELISA Test Kit
Catalog No. LSY-10026
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Melamine in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Melamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Melamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the Melamine concentration in the sample. This value is compared to the standard curve and the Melamine concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 1 ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit:
Milk powder................................................................................... 20 ppb
Milk............................................................................................... 16 ppb
Milk/Milk powder (Method two).......................................................... 1 ppb
Tissue (chicken, pork, duck, fish, shrimp and liver)............................. 2 ppb
Feed............................................................................................ 100 ppb
Egg............................................................................................... 20 ppb
Serum............................................................................................. 4 ppb
Cross-reaction rate:
Melamine......................................................................................... 100%
Cyanuric acid..................................................................................... 60%
Triazine.............................................................................................. <1%
Diamino atrazine................................................................................ <1%
Recovery rate:
Milk/Milk powder........................................................................... 90±19%
Tissue.......................................................................................... 85±20%
Feed............................................................................................ 85±20%
Egg.............................................................................................. 80±20%
3. Components
- Micro-well strips: 12 strips with 8 removable wells each
- 6× standard solution (1 mL each): 0 ppb,1 ppb, 3 ppb, 9 ppb, 27 ppb, 81 ppb
- Enzyme conjugate (7 mL)............................................................... red cap
- Antibody working solution (7 mL).................................................. blue cap
- Substrate A solution (7 mL).......................................................... white cap
- Substrate B solution (7 mL)......................................................... black cap
- Stop solution (7 mL).................................................................. yellow cap
- 20× concentrated washing buffer (40 mL)..................................... white cap
- 2× concentrated redissolving solution (50 mL)..................... transparent cap
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