Quick Detail
Place of Origin
HS-CODE
30-
Package & Delivery Lead Time
Detailed Description
Recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.
Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.
SOURCE:
E.Coli, HPLC purified.
SEQUENCE:
Protein A mutant of Staphylococcus aureus
MOL. WEIGHT:
25 kDa
FORM:
Lyophilized powder.
Solution: 50mg/ml in deionized water or PBS.
PH STABLE RANGE:
pH 1-13
STORAGE CONDITION:
-20°C or below.
PRODUCT SPECIFICATION
Bioburden No organisms detected.
12% SDS-PAGE Single major band
Coomassie Stain 25kD
UV Spectrum E270/250≥1.2, E0.1% at 270nm=0.14-0.20
UV spectrometry No DNA or RNA detected
Triton Content No Triton contaminant.