Recombinant Protein A CAS:91932-65-9

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Recombinant Protein A CAS:91932-65-9
Posting date : Apr 04, 2014
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Recombinant Protein A is a genetically engineering protein containing IgG-binding domains. Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form. Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests. SOURCE: E.Coli, HPLC purified. SEQUENCE: Protein A mutant of Staphylococcus aureus MOL. WEIGHT: 25 kDa FORM: Lyophilized powder. Solution: 50mg/ml in deionized water or PBS. PH STABLE RANGE: pH 1-13 STORAGE CONDITION: -20°C or below. PRODUCT SPECIFICATION Bioburden No organisms detected. 12% SDS-PAGE Single major band Coomassie Stain 25kD UV Spectrum E270/250≥1.2, E0.1% at 270nm=0.14-0.20 UV spectrometry No DNA or RNA detected Triton Content No Triton contaminant.

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