Hyaluronidase

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Hyaluronidase is extracted from the mammalian testicular to hydrolyse hyaluronic acid polysaccharides. The enzyme activity is not less than 300 units per mg. PREPARATION Hyaluronidase is extracted from the quarantine inspected mammalian testicular. The production process conforms to GMP requirement. CHARACTERISTICS White to light yellow powder, odourless, soluble in water but insoluble in ethanol, acetone or ether. PH At a concentration of 3mg/ml, the hyaluronidase solution pH is 4.5 ~ 7.5. COLOUR Weigh 0.1 g hyaluronidase and dissolve it in 10ml of water, the solution is clear and colourless. If colour appears, it will not be deeper than the 4th yellow standard solution. ABSORBANCE Weigh appropriate amount of hyaluronidase and dissolve it in water to 300 units/ml, measure the absorption according to spectrophotometry method. The measured absorbance is less than 0.6 at 280nm, and less than 0.42 at 260nm. DRY WEIGHT LOSS Weigh 0.1 g hyaluronidase and put it in P2O5 dessicator. Dry for 4h under low pressure, the weight loss is less than 5.0%. TYROSINE CONTENT No more than 0.1μg tyrosine is found in 1 unit hyaluronidase. ABNORMAL TOXICITY Conform to the standard of C.P. ENDOTOXIN Not more than 0.20 EU endotoxin is found in 1 unit hyaluronidase. ACTIVITY MEASUREMENT The activity is measured according to C.P. and the measured values are conformed to the C.P. (2010 edition, appendix XII (C)) CATEGORY Glycosaminoglycan decomposing enzyme. STORAGE Airtight, preserve in a cool and dry place.

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Shanghai Yaxin Biotechnology Co., Ltd.

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