Deoxynivalenol ELISA kit

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Deoxynivalenol ELISA kit
Posting date : Apr 02, 2014
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38-
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Before the start of the experiment, all reagents should be balanced to room temperature (reagent can not be directly dissolved in 37 ℃); reagent or sample dilution, the required mixing, mixing time to avoid foaming. Content of the sample to be predicted before the experiment, such as the sample concentration is too high, the sample should be diluted in order to meet the diluted sample kit for the detection range, the calculation multiplied by the corresponding dilution. 1. Adding sample: blank holes are located, standard hole, tested the sample hole. Blank wells plus sample diluent 100μl, and the remaining holes were standard or sample was 100μl, careful not have bubbles, add sample to sample additional hole in the bottom of microtiter plates, trying not to touch the wall hole. After that, gently shake mix, enzyme The panels covered or coated with, 37 ℃ 120 minutes reaction. To ensure the validity of experimental results, each experiment uses the new standard solution.

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