Swine Foot and Mouth Disease Distinguishing Test Kit

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Usage Swine Foot and Mouth Disease Distinguishing ELISA Test kit is used for detection the non-structural 3ABC-protein of swine foot and mouth disease (FMD-3ABC) antibodies in the serum, differentiation naturally infected from vaccined swine. Brief Introduction The test kit is made from the antigen coated microplate, enzyme conjugation(HRP goat-anti-pig IgG), and other reagents, it applies the indirect ELISA principle to test the antibodies against FMD-3ABC in the serum. Principle The test kit is the antigens coated microplate made through FMD-3ABC protein. In the test, the diluted control serum and sample are added, then incubate. If FMD-3ABC specific antibodies exist in the sample, it will be bound with the antigens of the microplate but the unbound antibodies and other components will be removed by washing. Next add enzyme conjugation (anti-pig IgG-HRP) to specifically bind with the compound of antibodies and antigens on the microplate. Then the unbound conjugations will be removed by washing, Add the TMB substrate in the well, react with HRP to a blue product. At last, end the reaction by adding stop solution.

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lvshiyuan biotechnology co.,ltd

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