Porcine Parvovirus ELISA test Kit

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Usage The porcine parvovirus ELISA test kit is used for the detection of porcine parvovirus antibodies in swine serum; assessment of immunity conditions against porcine parvovirus in the pig farm; investigation epidemiology of the porcine parvovirus. Brief Introduction The test kit is composed of the antigen coated microplate, HRP goat-anti-pig IgG, and other reagents; it applies the indirect ELISA principle to test the antibodies against porcine parvovirus in pig serum. Principle The test kit is the antigen coated microplate made through the inactivation, purification and concentration of the porcine parvovirus. In the test, the diluted control serum and sample are added, then incubate. If porcine parvovirus specific antibodies exist in the sample , it will be bound with the antigens on the microplate, remove the unbound antibodies and other components by washing. Add IgG-HRP to specifically bind with complex of antibody-antigens on the microplate. Then the unbound enzyme conjugate (Anti-pig IgG-HRP combination) will be removed by washing. Then add the TMB substrate to the wells, react with HRP to generate a blue color. Stop the reaction by adding HF solution, measure the OD630nm value.

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lvshiyuan biotechnology co.,ltd

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