Porcine Encephalitis Virus ELISA diagnostic kits

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1. Usage The porcine encephalitis virus ELISA test kit is used for the detection of the porcine encephalitis virus antibodies in swine serum; assessment the immunity conditions against porcine encephalitis virus in the pig farm; and investigation of the epidemiology of the porcine encephalitis virus. 2. Introduction The test kit is made from the antigen coated microplate, enzyme conjugations(HRP goat-anti-pig IgG), and other reagents. It applies the indirect ELISA principle to test the antibodies against porcine encephalitis virus in pig serum. 3. Principle The test kit is the antigen coated microplate made through the inactivation, purification and concentration of the porcine encephalitis virus. In the test, the diluted control serum and sample are added, then incubate. If porcine encephalitis Virus specific antibodies exists in the sample, It will be bound with the antigens on the microplate, but the unbound antibodies and other components will be removed by washing. Next, add enzyme conjugations(anti-pig IgG-HRP) to specifically bind with the compound on the microplate. Then the unbound conjugations will be removed by washing. Add the TMB substrate in the well, react with HRP to a blue product. At last, end the reaction by adding stop solution.

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lvshiyuan biotechnology co.,ltd

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