Nitrofuran (SEM) ELISA Test Kit

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1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of semicarbazide (SEM) in chicken, fish, shrimp, milk, honey, whole egg. The coupling antigens are pre-coated on the micro-well stripes. The SEM in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-SEM antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the SEM in it. This value is compared to the standard curve and the SEM concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.1 ppb Detection limit Tissue, honey, egg 0.2 ppb Fish, shrimp (some interference) 0.3 ppb Recovery rate Shrimp and fish 85±10% Honey ,chicken meat /liver,whole egg 75±15% Cross-reaction rate SEM 100% AHD ﹤0.1% AMOZ ﹤0.1% AOZ ﹤0.1%

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lvshiyuan biotechnology co.,ltd

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