Nitrofuran (AOZ) ELISA Test Kit

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1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of AOZ (3-anmino-2-oxazolidinone) in the meat, chicken, fish, shrimp and honey. The coupling antigens are pre-coated on the micro-well stripes. The AOZ in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AOZ antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with AOZ concentration in the sample. This value is compared to the standard curve and the content of the corresponding AOZ is subsequently obtained. 2. Technical specifications Sensitivity : 0.05 ppb Detection limit: Tissue and honey 0.1 ppb Shrimp,fish(some interference) 0.2 ppb Recovery rate: Shrimp and fish 90±10% Honey ,chicken meat /liver 75±15% Cross-reaction rate: AOZ 100% AHD <0.1% AMOZ <0.1% SEM <0.1%

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lvshiyuan biotechnology co.,ltd

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