Malachite green ELISA kit

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1. Principle This test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Malachite green in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Malachite green antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Malachite green in it. This value is compared to the standard curve and the content of Malachite green residues is subsequently obtained. 2. Technical specifications Sensitivity: 0.01 ppb Detection limit Shrimp, Fish 0.5 ppb Scientific metabolism principle and lots of screening positive sample statistics indicate: the Malachite green total residues of Malachite green and Latent Malachite green in the positive sample is equal to the Malachite green residues in the testing positive sample multiplied by 2.27. The total residues of Malachite green and Latent Malachite green (Yppb)= The testing Malachite green(Xppb)×2.27 Recovery rate Shrimp, Fish 80±10% Cross-reaction rate Malachite green 100% Latent Malachite green < 1% Crystal < 120% Latent Crystal < 1%

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lvshiyuan biotechnology co.,ltd

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