Florfenicol ELISA Test Kit

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Florfenicol ELISA Test Kit
Posting date : Jun 23, 2008
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16-
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Detailed Description
1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of Florfenicol in the tissue (chicken, pork), fish, shrimp and honey. The coupling antigens are pre-coated on the micro-well stripes. The Florfenicol in the sample and the conjugate antigens pre-coated on the micro-well stripes compete for the anti-Florfenicol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value has a negative correlation with the Florfenicol concentration in the sample. This value is compared to the standard curve and the Florfenicol concentration is subsequently obtained. 2. Technical specifications Sensitivity : 0.5 ppb Detection limit: Honey 1.5 ppb Meat, liver, shrimp, fish 0.5 ppb Recovery rate: Honey 70 ±10% Meat, liver, shrimp, fish 80 ±10% Cross-reaction rate: Florfenicol 100% Florfenicol Amine 11% Thiamphenicol < 0.1% Chloramphenicol < 0.1%

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