Chloramphenicol ELISA Kit

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Chloramphenicol ELISA Kit
Posting date : Jun 23, 2008
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04-
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Detailed Description
1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of Chloramphenicol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Chloramphenicol in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Chloramphenicol antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chloramphenicol in it. This value is compared to the standard curve and the Chloramphenicol concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.05 ppb Detection limit Egg, intestine 0.1 ppb Honey 0.15 ppb Milk 0.05 ppb Urine and serum 0.1 ppb Chicken, pork, fish, shrimp 0.05 ppb Recovery rate Chicken, pork, fish, shrimp 80%±10% Honey 70%±10% Milk 85%±15% Cross-reaction rate Chloramphenicol 100% Thiamphenicol <0.1% Florfeniol <0.1%

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