Studying gene functions in metabolic pathway or disease development is very important for disease mechanism research and drug discovery. Leading a new generation of gene editing technology, the CRISPR-Cas9 system is popular for its simplicity of operation and its universal applicability. Because of these unique advantages, CRISPR-Cas9 technology has become a powerful tool for forward genetic screening. Mutant libraries of various functions can be constructed by CRISPR, and the genes related to this function then identified through functional screening and enrichment, PCR amplification, and deep sequencing analysis.
Synbio Technologies provides one-stop gene function screening services based on the CRISPR-Cas9 sgRNA library system. With our proprietary Syno®3.0 DNA synthesis platform, we can construct a sgRNA library in a fast and efficient manner. We can also package the library into lentivirus, transfect cells, perform high throughput/content screening, and finally identify the target genes by sequencing and data analysis.
Screening resistance targets for a drug with identified function
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Service specifications
Synbio Technologies provides one-stop solution including sgRNA library design, synthesis, lentivirus packaging, and high throughput/content screening
Service Name | Service Specifications | Timeline | Deliverables |
CRISPR-Cas9 sgRNA lentivirus library (Human) | Targeting 21,000 genes; 6 sgRNAs for each gene | Inquiry | Lentivirus,-80 ℃ preservation |
customized CRISPR-Cas9 sgRNA library | Customized gRNA library, including genome-scale library, lncRNA library, cell apoptosis, cell proliferation, signal pathway, ion channel, nuclear receptor libraries | Inquiry | LyophilizedsgRNA plasmidSequencing report |
lentivirus packaging of sgRNA library | Customized sgRNA design, 6 sgRNA for each gene Lentivirus package | Inquiry | Lentivirus |
sgRNA library screening | cell screening using cell survival control, immunostaining, flow cytometry and other screening methods | Inquiry | Screening report and sequencing data |
How to order CRISPR Cas9
If you have any question, please contact us anytime for assistance in business days. Our experienced project managers will provide you professional support to ensure the success of your project.
Online Inquiry | service@synbio-tech.com | +1 732-230-3003 | +1 609 228 5911 |