Acrosome reaction occurs during period when the sperms are combined with the pellucid zone of the ovum and before sperms fertilize oocyte by penetrating the membrane of oocyte. The SpermFuncTM ARIC can evaluate the ability of sperm's acrosome reaction by the staining with fluorescence after sperm capacitation and acrosome reaction induced by calcium ionophore A23187 in vitro. The test result can provide the evidence for analysis of infertile pathogeny and selection of the treatment in ART.
When the calcium ionophore A23187 induces the Ca2+ into the inside of sperm, the acrosome reaction happened by the allosterism of sperm acrosome. Since there are glycoproteins in outer membrane of sperm acrosome which can be combined with the lectins specifically, the transformations of the zone in the sperm acrosome can be observed by staining with the lectin fluorescence so that we can evaluate the status of acrosome reaction in sperm.
SpermFuncTM ARIC is designed according to the method recommended by WHO laboratory manual for the examination and diposing of human semen (5th Edition). Treated with the industrial and systemed technology, the SpermFuncTM ARIC can make the testing process in Labs easy and the testing results reliable and repeatable.
Features:
1. The revolutionary design and the first product for determination of induced acrosome reaction by calcium ionophore A23187 in the world.
2. To provide more guarantees in accuracy and repeatability of testing results.
3. Using the sensitive and specific method of testing according to WHO criteria.
4. All the testing technique is proved by a systemic study.
5. All the components of this kit are under control of the strict quality.
6. The configuration of the zone in sperm acrosome after induction is typical and distinct and easy to be observed.
7. The background of fluorescence is clear and the intensity of fluorescence is high and stable for at least 15 days.
8. The operation of the SpermFuncTM ARIC is more humanized.
9. Overcome the disadvantages of long reaction time and high-intensity testing work in the previous method. The whole testing process is designed for two parts. After the process of sperm capacitation in vitro and the acrosome reaction induced by calcium ionophore A23187, the staining with the fluorescence can be done at anytime within 72 hours without affecting the final testing result. It can reduce the intensity of the work and enable batch testing.
10. All testing devices necessary for testing are equipped in the SpermFuncTM ARTC; Easy to use.
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China [CN] 
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