GZMB Assay/Inhibitor Screening Fluorometric Kit

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Product
GZMB Assay/Inhibitor Screening Fluorometric Kit
Posting date : Nov 14, 2017
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Free Member Scince Jan 12, 2015
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1
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1000000
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Candy Ton
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Place of Origin
United States [US]
Brand Name
Creative BioMart
HS-CODE
-
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XX
Detailed Description
Granzyme B (GZMB, EC number 3.4.21.79) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell. In GZMB Activity Assay Kit (Fluorometric), we have utilized a peptide substrate containing the Granzyme B recognition sequence along with a fluorescent label "AFC". Granzyme B catalyzes the cleavage of this substrate and releases the AFC molecule, which can be detected fluorometrically (Ex/Em = 380/500 nm). This Assay Kit is rapid, reliable and sensitive and can detect Granzyme B activity in various biological samples.
 

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