Human AECA ELISA Kit

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Human AECA ELISA Kit
Posting date : May 20, 2013
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Free Member Scince Aug 30, 2011
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Place of Origin
United States [US]
HS-CODE
29-
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Detailed Description
The kit assay Human AECA level in the sample,use Purified antigen to coat microtiter plate wells, make solid-phase antigen, then add AECA to wells, Combined antigen which With HRP labeled,become antigen-antibody- enzyme-antigen complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AECA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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