Human L-Lactate Dehydrogenase ELISA Kit

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Human L-Lactate Dehydrogenase ELISA Kit
Posting date : May 24, 2012
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Free Member Scince Aug 30, 2011
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Place of Origin
United States [US]
HS-CODE
29-
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Detailed Description
The kit assay Human L-LDH level in the sample,use Purified Human L-LDH antibody to coat microtiter plate wells, make solid-phase antibody, then add L-LDH to wells, Combined L-LDH which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of L-LDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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