Human Leishmania ELISA Kit

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Human Leishmania ELISA Kit
Posting date : May 24, 2012
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Free Member Scince Aug 30, 2011
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Place of Origin
United States [US]
HS-CODE
29-
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Detailed Description
The kit assay Leishmania level in the sample,use Purified Leishmania antibody to coat microtiter plate wells, make solid-phase antibody, then add Leishmania to wells, Combined With Leishmania, after washing and removing non-combinative antibody and other components ,then Combined Leishmania antibody which with HRP labeled become antibody - antigen - enzyme- antibody complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according to this to judge Leishmania exist in the sample or not.

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