Testosterone ELISA Test Kit

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Testosterone ELISA Test Kit
Posting date : Nov 10, 2011
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United States [US]
HS-CODE
21-
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Product Description REAGEN Testosterone ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of Testosteronein fish, shrimp, meat ,plasma and urine. The unique features of the kit are: Rapid (10-40min), high recovery (>80%), and cost-effective extraction methods . High sensitivity (0.05 ng/g or ppb) and low detection limit (0.05 ng/g or ppb) . A quick ELISA assay (less than 2 hours regardless of number of samples). High reproducibility. Procedure Overview The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample. Kit Contents, Storage and Shelf Life Testosterone ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C*. The shelf life is 12 months when the kit is properly stored. Kit Contents Amount Storage Testosterone-coated Microtiter Plate 1 x 96-well plate (8 wells x 12 strips) 2-8C Testosterone Standards: Negative control (white cap tube) 0.05ng/mL (yellow cap tube) 0.15 ng/mL (orange cap tube) 0.45 ng/mL (pink cap tube) 1.35 ng/mL (purple cap tube) 4.05 ng/mL (blue cap tube) 100 ng/mL (spiking, optional, red cap tube) 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 2-8C Testosterone Antibody #1 15mL 2-8C * 100X HRP-Conjugated Antibody #2 250 L 2-8C * Antibody #2 Diluent ** 20 mL 2-8C 10X Sample Extraction Buffer ** 25 mL 2-8C 20X Wash Solution ** 28 mL 2-8C Stop Buffer ** 20 mL 2-8C TMB Substrate ** 12 mL 2-8C Clean up mix 28ml 2-8C * If you are not planning to use the kit for over 3 months, store Antibody #1 and 100X HRP-Conjugated Antibody #2 at -20C or in a freezer. Sensitivity Sample Type Detection Limit (ng/g or ppb) Shrimp/Fish/Meat 0.05 Plasma/Serum 0.025 Urine 0.05 Specificity Analytes Cross-Reactivity (%) Testosterone 100 Methyltestosterone 0.9 Cortisol 0.1 Progesterone 0.4

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