Okadaic Acid（DSP）ELISA Test Kit

REAGEN™Okadaic Acid (DSP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Okadaic Acid (DSP) in mussels and water samples.

The method is based on a competitive colorimetric ELISA assay. The second antibody against Anti-Okadaic Acid Antibody was coated in the plate wells. During the analysis, sample is added along with the okadaic acid-horseradish peroxidase conjugate (Okadaic Acid-HRP Conjugate) and Anti- Okadaic Acid Antibody. If the okadaic acid residue is present in the sample, it will compete with Okadaic Acid-HRP Conjugate for the Anti-Okadaic Acid Antibody, thereby preventing the Okadaic Acid-HRP Conjugate from binding to the Anti-Okadaic Acid Antibody. The Anti-Okadaic Acid Antibody is then bound by the second antibody immobilized on the plate. After a washing step and addition of the TMB substrate solution, a color signal is produced. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The resulting color intensity has an inverse relationship with the okadaic acid residue concentration in the sample.